Plasmids

Synthetic phage display

Type

Applications

Price (USD)

pSyn.phage2
$200.00
  • A synthetic plasmid for phage display (phagemid)

  • Contains the full-length pIII of M13 phage

  • Amber stop codon between the gene of interest and pIII for phage display applications in suppersor bacteria (e.g. SupE in TG1 & ER2738)

  • Soluble expression in non-suppresor bacteria (e.g. SS320)

For phage display

$200

Antibody phage display, peptide phage display,

Phage ELISA, Phage western blot

pSyn.phage3
$200.00
  • A synthetic plasmid for phage display (phagemid)

  • Contains the full-length pIII of M13 phage

  • Gene of interest design to be fused with pIII for phage display applications in all bacteria expressing phage-competent pili (e.g. TG1, ER2738, SS320)

For phage display

$200

Antibody phage display, peptide phage display,

Phage ELISA, Phage western blot

pSyn1.C3-ScFv+
$200.00
  • A synthetic plasmid for phage display (phagemid) encoding for the C3-ScFv that targets HLA-CW7-Nef1 complex

  • Contains the N-terminal truncated pIII of M13 phage

  • Amber stop codon between the gene of interest and pIII for phage display applications in suppersor bacteria (e.g. SupE in TG1 & ER2738)

  • Soluble expression in non-suppresor bacteria (e.g. SS320)

Single chain fragment variable (antibody fragment)

targets HLA-CW7:02 presenting HIV-1 Nef1 peptide

$200

ScFv phage display, soluble ScFv expression

ELISA, Flow cytometry

Protein Expression

Type

Applications

Price (USD)

pExpress
$200.00

A plasmid for high levels transient or stable protein expression in mammalian cells.

The plasmid features the CMV promoter, WPRE stability element, puromycin resistance gene for selection of stably transfected clones, and ampicillin resistance gene for bacterial propagation.

The plasmid has been used for hundreds of different transfections of numerous protein-expression plasmids; it was successfully used for generating stable cell lines selected for puromycin resistance.

Transient / stable protein expression in mammalian cells

Expression of soluble secreted or intracellular proteins

$200

phAKT1
$200.00

Boost transient expression of proteins. An auxiliary plasmid that expresses a codon-optimized human RAC-alpha serine/threonine-protein kinase protein (human AKT1; uniprot entry P31749) in mammalian cells. AKT kinases regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis.

When phAKT1 is co-transfected with a second plasmid into cells, it promotes the proliferation and survival of the transfected cells.

The plasmid features the CMV promoter, WPRE stability element, Neomycin resistance gene for selection if needed, and ampicillin resistance gene for bacterial propagation.

Expression of RAC-alpha serine/threonine-protein kinase 1 (AKT1);

Enhances cell viability and growth during transient protein expression in mammalian cells

Co-transfection with protein-expression plasmids

$200

pLTag
$200.00

Boost transient expression of proteins from plasmids that contains the SV40 origin of replication.

An auxiliary plasmid that expresses a codon-optimized large T antigen (LTag) of simian virus 40 (SV40) in mammalian cells. When co-transfected with a second plasmid that contains SV40 origin of replication (SV40 ori), the LTag will bind SV40 ori and synthesize additional copies of the plasmid in the cell.

Thus, co-transfection with a SV40 ori-containing plasmid will increase the copy number of the plasmid and boost the protein expression from the related plasmid.

The plasmid features the CMV promoter, WPRE stability element, Puromycin resistance gene for selection if needed, and ampicillin resistance gene for bacterial propagation.

Expression of SV40 large T antige

Enhances transient protein expression from plasmids that contain the SV40 origin of replication

Co-transfection with protein-expression plasmids that contain the SV-40 origin of replication

$200

Pathway / network libraries (coming soon)

Type

Applications

Price (USD)

Interferon stimulating genes

Human ISGs

Screening ISGs

Studying the effects of single or defined combination of ISGs

From $200